KMID : 0613820190290070809
|
|
Journal of Life Science 2019 Volume.29 No. 7 p.809 ~ p.816
|
|
Anti-proliferative Properties of p-Coumaric Acid in SNU-16 Gastric Cancer Cells
|
|
Jang Mi-Gyeong
Ko Hee-Chul Kim Se-Jae
|
|
Abstract
|
|
|
The ubiquitous plant metabolite p-coumaric acid (p-CA) has antioxidant and anti-inflammatory properties, but its anti-cancer activity has not been established in gastric cancer cell lines. In this study, we investigated the effects of p-CA on the proliferation and transcriptome profile of SNU16 gastric cancer cells. Treatment with p-CA induced apoptosis of the SNU-16 cells by regulating the expression of pro-apoptotic and anti-apoptotic proteins, such as Bcl-2, poly (ADP-ribose) polymerase (PARP), Bax, procaspase-3, and cleaved-caspase-3. The genes differentially expressed in response to p-CA treatment of the SNU-16 cells were identified by RNA sequencing analysis. Genes regulated by p-CA were involved mainly in the inflammatory response, apoptotic processes, cell cycle, and immune response. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that the phosphatidylinositol-3-kinase-Akt and cancer signaling pathways were altered by p-CA. Protein-protein interaction (PPI) network analysis also revealed that p-CA treatment was correlated with differential expression of genes associated with the inflammatory response and cancer. Collectively, these results suggest that p-CA has potential utility in gastric cancer prevention.
|
|
KEYWORD
|
|
Gastric cancer cells, p-coumaric acid, RNA-sequencing, transcriptome
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|